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Dr. Jyoti Gupta

Dr. Jyoti Gupta

  • Assistant Professor
    Institute of Applied Sciences & Humanities

  • Department

    Department of Biotechnology

  • Contact Details:

    Email : jyotigupta15dec@gmail.com

    Contact Number :

  • Qualifications

    • Ph.D (2016) Academy of scientific and Innovative Research, New Delhi, India Title: “Evaluation of Immunoprophylactic Efficacy of DNA Vaccine Employing Brugia malayi Heavy Chain Myosin (Bm-Myo) and Trehalose-6-Phosphate Phosphatase (Bm-TPP)” Supervisor: Dr. Shailja Bhattacharya, Central Drug Research Institute, Lucknow India.
    • Master of Technology in Biotechnology (2010), UP Technical University, Lucknow, Uttar Pradesh.
    • Master of Sciences in Biochemistry (2005), CSJM, University, Kanpur, Uttar Pradesh.
    • Bachelor of Sciences (2003) CSJM, University, Kanpur, Uttar Pradesh.
    • Intermediate (2000) UP Board
    • Xth Standard (1998) UP Board

Publication

  • 01Shabnam Ansari, Nidhi Gupta, Rohit Verma, Oinam N Singh, Jyoti Gupta, Amit Kumar, Mukesh Kumar, Yadav, Akshay, Binayke Mahima Tiwari, Neha Periwal, Vikas Sood, Shailendra Mani Amit Awasthi Shalimar Baibaswata Nayak, CT Ranjith-Kumar, Milan Surjit. Antiviral activity of the human endogenous retrovirus-R envelope protein against SARS-CoV-2. 2023, EMBO Reports, e55900
  • 02Jyoti Gupta , Minnah Irfan , Niranjan Ramgir , K P Muthe , A K Debnath , Shabnam Ansari , Jaya Gandhi , C T Ranjith-Kumar , Milan Surjit. Antiviral Activity of Zinc Oxide Nanoparticles and Tetrapods Against the Hepatitis E and Hepatitis C Viruses. 2022, Frontiers in microbiology. 10.3389/fmicb.2022.881595
  • 03Jyoti Gupta, Sheetal Kaul, Akriti Srivastava, Sukanya Ghosh, Chandresh Sharma, Manidipa banerjee, Shalimar, Baibaswata Nayak, C. T. Ranjith-Kumar, Milan Surjit. Expression and characterization of Hepatitis E virus like particle (VLP) expressed in Pichia pastoris. 2020, Frontiers in microbiology. doi.org/10.3389/fmicb.2020.00141
  • 04Jyoti Gupta, Amit Kumar & Milan Surjit. Production of a Hepatitis E Vaccine Candidate Using the Pichia pastoris Expression System, 2021, Methods in molecular Biology protocols, 2415, 117-131
  • 05Jyoti Gupta, Manisha Pathak, Sweta Misra and Shailja Misra-Bhattacharya. CpG enhances the immunogenicity of heterologous DNA prime/protein boost vaccination employing heavy chain myosin of Brugia malayi in BALB/c mice. 2019, Parasitology Research doi.org/10.1007/s00436-019-06318-6.
  • 06Saumya Anang, Nidhi Kaushik, Smita Hingane, Anita Kumari, Jyoti Gupta, Shailendra Asthana, Shalimar, Baibaswata Nayak, C.T. Ranjith-Kumar, Milan Surjit. Potent inhibition of hepatitis E virus release by a cyclic peptide inhibitor of the interaction between viral open reading frame3 protein and host tumor susceptibility gene 101. 2018, Journal of virology,92(20), e00684-18.
  • 07Sweta Misra, Jyoti Gupta and Shailja Misra-Bhattacharya. RNA interference mediated knockdown of Brugia malayi UDP-Galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae.2017, Parasite and Vector, DOI 10.1186/s13071-017-1967-1.
  • 08Jyoti Gupta, Sweta Misra and Shailja Misra-Bhattacharya Immunization with Brugia malayi myosin as heterologous DNA prime protein boost induces protective immunity against B. malayi infection in Mastomys coucha. 2016, PLoS One 11(11), e0164991.
  • 09Sweta Misra, Guru Valicherla, Mohd Shahab, Jyoti Gupta, Jiaur Gayen, Shailja Misra-Bhattacharya. UDP-Galactopyranose mutase, a potential drug target against human pathogenic nematode B. malayi. 2016, Pathogens and Disease, 74(6) Doi. http://dx.doi.org/10.1093/femspd/ftw072
  • 10Jyoti Gupta, Manisha Pathak, Swera Misra and Shailja Misra-Bhattacharya. Immunogenicity and Protective Efficacy of Brugia malayi Heavy Chain Myosin as Homologous DNA, Protein and Heterologous DNA/Protein Prime Boost Vaccine in Rodent Model. 2015, PLoS One 10, e0142548.
  • 11Rahul Shukla, Jyoti Gupta, Prasant Shukla, P. Dwivedi, P. Tripathi, Shailja M. Bhattacharya and Prabhat R. Mishra. Chitosan coated alginate micro particles for the oral delivery of antifilarial drugs and combinations for intervention in Brugia malayi induced lymphatic filariasis. 2015, RSC Adv, 5, 69047-69056.
  • 12Sweta Misra, Lav Kumar Singh, Priyanka, Jyoti Gupta, Shailja Misra-Bhattacharya, Diksha Katiyar. Synthesis and biological evaluation of 4-oxycoumarin derivatives as a new class of antifilarial agents. 2015, European Journal of Medicinal Chemistry, 4, 211-17.
  • 13Koneni V. Sashidhara, Srinivasa Rao Avula, Pawan Kumar Doharey, L. Ravithej Singh, Vishal M. Balaramnavar, Jyoti Gupta, Shailja Misra-Bhattacharya, Sushma Rathaur, Anil K. Saxena, Jitendra Kumar Saxena. Designing, synthesis of selective and high-affinity chalcone-benzothiazole hybrids as Brugia malayi thymidylate kinase inhibitors: In vitro validation and docking studies. 2015, European Journal of Medicinal Chemistry, 20, 103, 418-28.
  • 14Jankiprasad Gunaganti Naresh, Jyoti Gupta,Shailja M. Bhattacharya, Siron M. Rajendran, Shailendra K. Awasthi and Tadigoppula Narender. Antifilarial Activity of Constituents of Calophyllum inophyllum and their Derivatives. 2013, Natural product communications, 8(6):803-804.
  • 15Jyoti Gupta, Sweta Misra, Sunil Kumar Mishra, Shishir Srivastava, M.N. Srivastava, Vijai Lakshmi, Shailja Misra-Bhattacharya Antifilarial Activity of Marine sponge Haliclona oculata against experimental Brugia malayi. 2012, Experimental parasitogy, 130(4), 449-55.
  • 16Jeetendra Kumar Nag, Nidhi Shrivastava, Jyoti Gupta., and, Shailja Misra- Bhattacharya. Recombinant translation initiation factor-1 of Wolbachia is an immunogenic excretory secretory protein that elicits Th2 mediated immune protection against Brugia malayi. 2012 Comparative immunology, microbiology and infectious diseases, 36, 25-38.
  • 17Sushila Kushwaha, Prashant Kumar Singh, Jyoti Gupta,Vishal. Kumar Soni., and Shailja Misra-Bhattacharya. Recombinant trehalose-6-phosphate phosphatase of Brugia malayi cross-reacts with human Wuchereria bancrofti immune sera and engenders a robust protective outcome in mice. 2012, Microbes and Infection 14, 1330-1339.
  • 18Koneni V. Sashidhara, Suriya P. Singh, Sweta Misra, Jyoti Gupta , Shailja Misra-Bhattacharya. Galactolipids from Bauhinia racemosa as a new class of antifilarial agents against human lymphatic filarial parasite, Brugia malayi. 2012, European Journal of Medicinal Chemistry, 50, 230-5.

Paper Presented In Conference

  • 01.Jyoti Gupta and Milan Surjit. Exploring the antiviral potential of Zinc Oxide nanoparticles and tetrapods against the Hepatitis E and Hepatitis C viruses. “International Union of Biochemistry and Molecular Biology-2022 at Reginal center for Biotechnology, Faridabad, India.
  • 02Jyoti Gupta, Sheetal Kaul, Manidipa Banerjee, Milan Surjit. Purification of a recombinant Hepatitis E virus-like particle (VLP) expressed in the Pichia pastoris. “6th molecular virology meeting 2019 at IIT Kharagpur,India.
  • 03Jyoti Gupta, Manisha Pathak, Sweta Misra and Shailja Misra-Bhattacharya.CpG enhances the immunogenicity of heterologous DNA prime/protein boost vaccination employing heavy chain myosin of Brugia malayi in BALB/c mice.“Current Trends in Drug Discovery and Research”- 2015 at CSIR- Central Drug Research Institute, Lucknow, India
  • 04Jyoti Gupta, Manisha Pathak, Sweta Misra and Shailja Misra-Bhattacharya. The immunoprophylactic efficacy of Brugia malayi adult female heavy chain Myosin (BmAF-Myo) as a DNA and heterologous prime boost vaccine in a rodent model.“National Congress of Parsitology”- 2014 at CSIR- Central Drug Research Institute, Lucknow, India
  • 05Jyoti Gupta, Sushila Kushwaha, Prasant kumar singh, Vishal kumar Soni and Shailja Misra-Bhattacharya Immunogenicity of Recombinant Trehalose-6-phosphate Phosphatase of Brugia malayi in BALB/c mice.“Current Trends in Drug Discovery and Research” - 2015 at CSIR- Central Drug Research Institute, Lucknow, India
  • 06Jyoti Gupta, Sushila Kushwaha, Prasant kumar singh, Vishal kumar Soni and Shailja Misra-Bhattacharya. Differential Immunoprophylactic efficacy of Withania somnifera chemotypes NMITLI 101R,NMITLI 118R, NMITLI, 128R and Withaferin A against filarial parasite Brugia malayi in Mastomys coucha. “Indian Society of Chemists and Biologists” -2013 at Mohanlal Sukhadia University, Udiapur
  • 07Jyoti Gupta, Sweta Misra, Sunil Kumar Mishra, Shishir Srivastava, M.N. Srivastava, Vijai Lakshmi, Shailja Misra-Bhattacharya The marine sponge Haliclona oculata possesses antifilarial activity against experimental human lymphatic filarial parasite Brugia malayi in rodent model “Immunocon”-2011 at University of Jammu

Workshops

  • 01Beckman Coulter Flow Cytometry Workshop 2014- CDRI, LKO

Technical Expertise

  • Molecular Biology:
    Gene cloning in different expression vectors, PCR, Fusion PCR, qRT PCR,
  • Biochemical techniques:Hands on experience in different protein expression systems and protein purification techniques like affinity, anion exchange and size-exclusion chromatography. Biochemical characterization of proteins, ELISA, western blotting and immunoprecipitation. Generation of polyclonal nonspecific antibodies and Immuno-precipitation.
  • Immunological techniques:Multicolor Flow cytometry assay, cytotoxic T cell assay, ELISA, lymphocyte proliferation assay
  • Animal handling:BALB/c mice, Mastomys, Gerbils and Rabbits were handled and have expertise in worm transplantation.
  • Tissue culture:Handling of different cell lines, virus isolation, reporter assay, BSL3 expertise

Awards And Fellowships

  • SERB – NPDF fellowship from Department of Science and Technology, India, 2017.
  • DBT-Research Associate fellowship from Department of Biotechnology, India, 2016.
  • Senior Research Fellowship from Council of Scientific and Industrial Research, India, (2013- to 2016).
  • Qualified GATE – 2006 with 93 percentile and availed GATE fellowship during M.Tech course.
  • Qualified Council of Scientific and Industrial Research’s National Eligibiligty Test for Lecture ship (CSIR NET LS 2009).

Contribution to Science

  • Postdoctoral Career:
    My post doctoral work was focused on vaccine development against HEV, In brief we have develop virus like particle using recombinant truncated ORF-2 protein of Hepatitis E virus, which encodes multi-epitope regions that may provide protective efficacy against HEV and could be a vaccine candidate. We have used yeast (P.pastoris) and bacterial ( E.coli) expression system for VLP production. In another project, I analysed the mechanism of Zinc underlying the inhibiting the replication of HEV and our investigation showed that zinc nanoparticles inhibit the replication of HEV and HCV and may use as a therapeutic candidate against RNA virus. Currently, I am also involved in a project of vaccine development against SARS-cov2 in which we are developing different formulations and evaluating the efficacy of vaccine formulations.
  • Graduate Career:My graduate research contributions were focused on DNA vaccination against lymphatic filariasis. Results from my research were highly relevant as they provided that heterologous DNA prime protein boost vaccination is a effective method of vaccination which could enhance more host immune response as compared to only protein and homologous DNA vaccination and further studies also showed that use of CpG adjuvant with DNA vaccination may also contribute to provide immunity against LF.

References

  • Dr. Shailja Bhattacharya Ex-Chief Scientist
    Parasitology Division,
    Central Drug Research Institute,
    Lucknow-226031
    Email: shailja_cdri@rediffmail.com
    Mob no. 9415010721
  • Dr. Milan Surjit Professor,
    Vaccine and Infectious Disease Research Centre,
    Translational Health Science and Technology Institute,
    3rd Milestone,
    Faridabad-121001, Haryana, india. Email: milan@thsti.res.in
    +91 129 2876 318
  • Dr. Mrigank Srivastava Sr. Scientist E
    Parasitology Division,
    Central Drug Research Institute,
    Lucknow-226031 Email: mrigank_srivasta@cdri.res.in
    Mob no. 0522-2772450

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