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Dr. Reetesh Kumar

Dr. Reetesh Kumar

  • Assistant Professor
    Institute of Applied Sciences & Humanities
  • Department

    Faculty of Agricultural Sciences
  • Contact Details:

    Email :

    Contact Number :+91-9369736596

  • Qualifications

    • Ph.D in Genetics and Plant Breeding on the topic "Protein folding problems - Physicochemical studies of proteins from Euphorbia cotinifolia and Streblus asper" in Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India [2005 – 2011]
    • Post graduation in Genetics and Plant Breeding, Allahabad Agriculture Institute - Deemed University, India 2004
    • Graduation in Science with 1st Class, Harish Chandra Post Graduate College, VBS University, India, 2002

4 Research Papers (as 1st or 2nd or corresponding Author excluding PhD Scholar)

  • 01Exploring the Binding Mechanism of Flavonoid Quercetin to Phospholipase A2: Fluorescence Spectroscopy and computational Approach Reetesh Kumar, Caruso, I P, Ullah A, Cornélio, M L, Fossey, M A, Souza, F P, Arni, RK. European J Experimental Biology. 7: 1-10, 2017. DOI: 10.21767/2248-9215.100033 (Impact factor - 3.312)
  • 02Reetesh Kumar, Tiwari S. Structure Insights and Fibrinogen peptides Molecular recognition by Bothrombin, A Snake Venom Serine Protease from Bothropus jararaca. European J Experimental Biology. 7: 1-7. 2017. DOI: 10.21767/2248-9215.100032 (Impact factor – 3.312)
  • 03Identification of folding intermediates of Streblin, the most stable serine protease: Biophysical analysis. Reetesh Kumar, Tripathi, P, Moraes, FR, Caruso IP, and Jagannadham, MV. Applied Biochemistry and Biotechnology 172 (2): 658 – 671, 2014. (Impact factor -2.926)
  • 04Evidence for a Molten Globule State in Cicer α-Galactosidase induced by pH, Temperature, and Guanidine Hydrochloride. Singh N, Reetesh Kumar, Jagannadham MV, Kayastha AM. Applied Biochemistry and Biotechnology, 169 (8): 2315-2325, 2013. (Impact factor – 2.926)

Other publications Published

  • 01Crinumin, a chymotrypsin-like but glycosylated serine protease from Crinum asiaticum: Purification and physicochemical characterization. Singh KA, Reetesh Kumar, Rao GRK, Jagannadham MV. Food Chemistry, 119: 1352-1358, 2010. (Impact factor – 6.306)
  • 02Biochemical and spectroscopic characterization of a novel metalloprotease, cotinifolin from an antiviral plant shrub: Euphorbia cotinifolia. Reetesh Kumar, Singh KA, Tomar R, Jagannadham MV. Plant Physiology and Biochemistry, 49: 721-728, 2011. (Impact factor – 4.26)
  • 03Assesment of Genetic Diversity and its usefulness for varietal identification in Indian elite varieties of Wheat (T. aestivum L. emthaell.) Using RAPD markers. Reetesh Kumar, Jaiswal SK, Vishwakarma MK, Rai VP, Upadhyay P, Joshi AK. Asian Journal of Biotechnology, 3: 460 - 469, 2011. (Impact factor - NA)
  • 04Biochemical characterization of a peroxidase isolated from Caribbean plant: Euphorbia cotinifolia. Reetesh Kumar, Singh KA, Singh VK, Jagannadham MV. Process Biochemistry, 46: 1350-1357, 2011. (Impact factor – 3.757)
  • 05Muthuramalingam P, Krishnan, S.R., Saravanan, K, Mareeswaran, N, Reetesh Kumar, Ramesh, M. Genome-wide identification of major transcription factor superfamilies in rice identifies key candidates involved in abiotic stress dynamism. Journal of Plant Biochemistry and Biotechnology. 27 (3), 300–31, 2018. DOI: ORG/10.1007/S13562-018-0440-3 (Impact factor – 1.175)
  • 06Purification and biochemical characterization of an alkaline pectin lyase from Fusarium decemcellulare MTCC 2079 suitable for Crotolaria juncea fiber retting. Yadav, S, Dubey, AK, Anand, G, Reetesh Kumar, Yadav, D. Journal of Basic Microbiology, 53: 1–9, 2013. (Impact factor – 2.281)
  • 07A Stable Serine Protease, Wrightin, from the Latex of the Plant Wrightia tinctoria (Roxb.) R. Br.: Purification and Biochemical Properties. Tomar R, Reetesh Kumar, Jagannadham MV. Journal of Agriculture and Food Chemistry, 56 (4): 1479-1487, 2008. (Impact factor – 4.192)


  • 01Impact of regulative noise exposure to biodiesel production due to enhanced lipid droplet production in Saccharomyces cerevisiae: Preliminary results from a laboratory experiment, Reetesh Kumar (corresponding author), Savitri Tiwari, Lakhvinder singh to Applied Microbiology and Biotechnology
  • 02In-silico evaluation of natural compounds to confirm their anti-DNA gyrase activity against Staphylococcus aureus, Seemab Siddiqui, Somnath Maji, Pandiyan Muthuramalingam, Hyunsuk Shin, Lakhvinder singh, Rajeev K. Tyagi, Dinesh Kumar Prajapati, Reetesh Kumar (corresponding author) to Applied Microbiology and Biotechnology
  • 03Autophagy circumvents anti P. falciparum defense under oleuropein pressure, Praveen Sharma, Sheetal Saini, Rajinder Kumar, Sushmita Negi, Reetesh Kumar, Bharat Singh Chandel, Puran S. Sijwali, Rajeev K. Tyagi to iscience

Conferences/ Symposiums

  • 01Attended Bioinformatics seminar in Mahila Mahavidyalaya, Banaras Hindu University, Varanasi, INDIA
  • 02Attended 5th Indian Agricultural Scientists and Farmer’s Congress in Allahabad University, Allahabad, INDIA
  • 03Attended a symposium on “The National Symposium on cellular and molecular biophysics” from CCMB, Hyderabad, AP, INDIA (22/01/2009 – 24/01/2009)
  • 04Attended a symposium on “Recent trends in biophysics and workshop on emerging techniques of biophysics” (February 13-16, 2010, BHU, Varanasi) INDIA

Visits Overseas

  • 01Visiting Post Doctoral fellow, University of Pompeu Fabra, Barcelona, SPAIN, 2015 - 2016
  • 02Worked as Postdoc fellow at USP, Sao Carlos, Sao Paulo, BRAZIL, 2014 - 2015
  • 03Interim training, Department of Neuroscience, University of Wisconsin, Madison, USA, 2014
  • 04Worked as Postdoc fellow at UNESP, Sao Jose do Rio Preto, Sao Paulo, BRAZIL, 2012 - 2013
  • 04Interim training, Department of Biophysical Chemistry, University of Groningen, Groningen, THE NETHERLANDS, 2009

Research Area

  • Structural biology, protein-ligand (Fluorescence quenching experiment and bioinformatics approach) and protein-protein interaction (bioinformatics approach), protein folding-unfolding analysis using Circular Dichroism and Fluorescence spectroscopy, Protein purification, Molecular Cloning

Technical expertise

  • Protein purification using ion exchange, affinity, gel filtration, hydrophobic interaction chromatography, ultra-centrifugation and ultra-filtration
  • Enzyme kinetics, inhibition kinetics and ligand binding studies.
  • Protein conformational studies using biophysical tools such as circular dichroism, fluorescence spectroscopy, Absorption spectroscopy.
  • Molecular Cloning and protein expression
  • Fluorescence quenching, protein-protein interaction studies along with bioinformatics tools, such as Modeller, Autodock, tinker etc.
  • Proficiency in, Image analysis software, Origin, Sigma Plot
  • Bioinformatics tools in proteomics, e.g. BLAST, PSI-BLAST, Clustalw, HMM (Hidden Markov Models), STAMP (Structural Alignment of Multiple Poteins), MODELLER, AUTODOCK, Statics Potential tools; e.g PROSA, Threading etc.
  • Proficiency in Windows and Linux OS
  • Drug Designing; Virtual Screening, Dynamic docking, Molecular Simulation using Gromacs
  • Some tools on Machine learning (e.g. Principal Component Analysis)

Academic Awards and achievements

  • CEMS (Centre of Experimental Medicine and Surgery) Research fellowshipINDIA
  • CSIR-SRF (Council for Scientific and Industrial Research) fellowshipINDIA
  • BIF (Boehringer Ingelheim Fonds) fellowship, Department of Biophysical chemistry, University of Groningen,NETHERLANDS
  • Wood Whelan Research Fellowship, Instituto de Quimica, Universidad Nacional autónoma de méxico (UNAM), (received but denied)MEXICO
  • CAPES (Coordenacao de aperfeicoamento de pessoa de nivel superior) postdoctoral fellowship, Department of Physics,Universidade Estadual Paulista (UNESP), Sao Jose do Rio Preto, Sao paulo,BRAZIL
  • FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) Postdoctoral fellowship Universidade de São Paulo–USP, SP,BRAZIL
  • BEPE (Bolsa Estágio de Pesquisa no Exterior) fellowship, University Pompeu Fabra (UPF), Barcelona,SPAIN
  • DS Kothari Fellow, Department of Biochemistry, Panjab University, Chandigarh,INDIA

Previous Experience/position

  • Principal Project Associate at Department of Biotherapeutics, CSIR-Institute of Microbial Technology (IMTech), Chandigarh – INDIA. Establishment of National Repository of GMP cell lines for Biopharmaceutical Products (NRGCBIO) [2020- 2022] INDIA
  • DS Kothari Post-Doctoral research fellow at Department of Biochemistry, Panjab University, Chandigarh, INDIA. Stress Granules assembly: Structural and biochemical characterization. The primary work based on drug designing on LSM protein, which caused pancreatic cancer. Teaching (Theory as well as practical) at the department, in addition. [2016 – 2019] INDIA
  • Post-Doctoral fellow; BEPE (Bolsa Estágio de Pesquisa no Exterior) fellowship at University Pompeu Fabra (UPF), Barcelona, SPAIN. Physicochemical characterization and protein-protein interaction analysis between pab1, pbp1 and pbp4 of stress granules proteins. The major work was based on basic bioinformatics techniques, such as sequence and structure alignment of multiple proteins with BLAST, HMMER and STAMP, Comparative homology modelling with MODELLER, and statistical potential analysis with Prosa. [2015 - 2016] SPAIN
  • Post-Doctoral Fellow; FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo) at Institute of Chemistry, Sao Carlos, University of Sao Paulo (USP), BRAZIL. Biochemical and structural characterization of assembling stress granules: The pros and cons of translation [2014 - 2015] BRAZIL
  • Interim training; Department of Neuroscience, University of Wisconsin, Madison, USA. Cloning and introducing mutations in a voltage-gated potassium channel. In addition, cloned an accessory subunit of calcium channel in baculovirus vector [July - September 2014] USA
  • Post-Doctoral Fellow; CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) at Department of Physics, Universidade Estadual Paulista (UNESP), São José do Rio Preto, Sao Paulo, BRAZIL. Post-translational modifications of venom proteins; protein-protein and protein-ligand interactions [2012 -2013] BRAZIL
  • Interim training; BIF (Boehringer Ingelheim Fonds) fellowship, Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands. Interim training during Doctorate (Ph.D.) times on the subject of Macromolecular crystallography (work on crystallization trials on the purified protease; Cotinifolin) [April - July 2009] NETHERLANDS

Thesis supervisor (no. 1)

  • Ph.D. Co-Supervisor – Mrs. Seemab Siddhiqui, Department of Biotechnology, Invertis University, Bareilly, UP, INDIA
  • Lecture on Structural Bioinformatics tools for proteomics data interpretation, 8 May 2020, Invertis University Bareilly, Uttar Pradesh, India
  • Protein-protein interaction lecture on Computer aided drug designing course 27 May - 2 June 2016 Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, UP, India

External examiner (no. 2)

  • External Examiner (M.Sc. Biotechnology / Molecular Biology), 18 December 2017, Invertis University Bareilly, Uttar Pradesh, India
  • External Examiner (M.Sc. Biotechnology), 28 May 2018, Invertis University Bareilly, Uttar Pradesh, India

Organizer (no. 1)

  • Organizer for a national level lecture-cum-practical training programme in Biochemistry 8-12 February, 2017, Department of Biochemistry, Panjab University, Chandigarh, India

Grant (no. 3)

Completed: 2

  • Bolsa no Exterior – Governed by FAPESP, Brazil on title “Physicochemical characterization and protein-protein interaction analysis between pab1, pbp1 and pbp4 of stress granule assembly” Grant Number: 15/07263-2, Amount: (63206.60 Brazilian Reals plus 32306.23 USD = Approx. 35 lakh INR), Duration: 9 months (2014-2015), Place: University Pomeu Fabra, Barcelona, SPAIN.
  • DS Kothari Fellowship – Governed by UGC, INDIA on title “stress granule assembly: structural and biochemical characterization” Grant Number: BL 15-16/0340, Amount: 2177808.00 INR, Duration: 3 Years (2016-2019), Place: Panjab University, Chandigarh, INDIA.

Submitted: 1

  • DBT grant on title “in silico decoding of e-waste degrading microbial metabolites for in vitro validation and utilization”. TRN: BT/PR/45754. Amount: 5529610.00 INR, Duration: 3 Year, Place: GLA University, Mathura, UP, INDIA


  • Dr. Bauke W. Dijkstra
    Em. Professor of Biophysical Chemistry Laboratory of Biophysical Chemistry,
    University of Groningen Nijenborgh 7,
    9747 AG Groningen,
    the Netherlands E-mail:
    Phone: +31-503634381 / fax: +31-503634800
  • Dr. Baldo Oliva Miguel Professor & Head
    Structural Bioinformatics Laboratory (GRIB-IMIM)
    Universitat Pompeu Fabra,
    Barcelona Research Park of Biomedicine (PRBB) C/ Doctor Aiguader,
    80., Barcelona-08003, Catalonia,
    Spain (EU) Email:
    Phone: +34-933160509 Fax: +34-933160550