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Dr. Saurabh Gupta

  • Assistant Professor- Research
    Institute of Applied Sciences & Humanities
  • Department

    Department of Biotechnology
  • Contact Details:

    Email : saurabh.gupta@gla.ac.in

    Contact Number :9368157137

  • Experience

    9.5 Years

  • Qualifications

    S.No Exam Board/University Subjects Year of passing Percentage
    1. Ph.D.* G.L.A. University, Mathura Biotechnology 2017 (Awarded)
    2. M.Sc. Ch. Charan Singh University, Meerut Biotechnology 2008 78.9
    3. B.Sc. Dr. B.R. Ambedkar University, Agra Botany, Chemistry, Zoology 2006 70.7
    4. Intermediate C.B.S.E. Biology, Mathematics, Chemistry, Physics, English 2003 68.6
    5. High School C.B.S.E. English, Hindi, Mathematics, Science, Social Science 2001 68.2

    *Ph.D. thesis title: Studies on Immunogenicity of secretory proteins of Mycobacterium avium subspecies paratuberculosis

    Guide Name: Dr. A.K. Bhatia, Professor Emeritus, GLA University, Mathura

  • Ph.D

    Thesis Supervised

  • Awarded/ Completed-

    Working-

  • Postgraduate

    Thesis Supervised

  • Awarded/ Completed-

    Working-

Publications: 75
(SCI indexed research articles: 20; Scopus Indexed research articles: 28
Others articles (NAAS, ICV, ESCI, ISI, UGC listed journals): 27

  • 1 Gupta S, Singh SV and Bhatia AK. 2017. Revisited Immuno-Reactivity between Native Semi-Purified Protoplasmic (Caprine) Versus Commercial Purified Protoplasmic (Bovine) Antigens for the Screening of Goatherds Endemic for Johne's Disease. Indian Journal of Biotechnology, 16(1): 22-29.
  • 2 Gupta S, Singh SV and Bhatia AK. 2016. Immuno-reactivity pattern of secretory proteins of Mycobacterium avium subspecies paratuberculosis vaccine strain 'S 5' with potential for diagnosis of Johne's disease in early infection. Indian Journal of Biotechnology, 15(3): 306-312.
  • 3 Gupta S, Singh SV, Gururaj K, Chaubey KK, Singh M, Lahiri B, Agarwal P, Kumar A, Misri J, Sohal JS, Hemati Z and Bhatia AK. 2017. Comparative evaluation of traditional IS900 PCR assay with newer assays ('Taqman probe PCR' and 'SYBR green Real time PCR') in thyroid disorder patients sero-positive for Mycobacterium avium subspecies paratuberculosis using Indigenous ELISA kit. Indian Journal of Biotechnology, 16(2): 228-234.
  • 4 Gupta S, Chaubey KK, Singh SV, Bhatia AK, Kumar N, Goel A, Sachan TK, Rawat KD, Sohal JS, Dhama K 2015. Immunoreactivity to culture filtrate proteins of Mycobacterium avium subspecies paratuberculosis in naturally infected goat and sheep sera. Advances in Animal and Veterinary Sciences, 3(6): 347-353.
  • 5 Gupta S, Singh SV and Bhatia AK. 2017. Sero-reactivity prototype of secreted proteins of native 'S 5' vaccine strain to human sera positive for Mycobacterium paratuberculosis infection. Journal of Experimental Biology and Agricultural Sciences, 5(2): 188-194.
  • 6 Khandelwal, V, Choudhary, PK, Goel, A, Bhatia, AK, Pant, G, Chaubey, KK, Gupta, S, Singh, SV. 2019. Cytokines modulating potential of Neolamarckia cadamba (Roxb.) Bosser. Indian Journal of Traditional Knowledge, 18(1): 88-93.
  • 7 Singh, M., Singh, S.V., Gupta, S., Chaubey, K.K., Stephan, B.J., Sohal, J.S., Datta, M. 2018. A novel 'Nano-immuno test' for the detection of live Mycobacterium avium subspecies paratuberculosis using magnetic nano-particles and chromogen. Veterinary Research Communications, DOI: 10.1007/s11259-018-9721-5.
  • 8 Khandelwal V., Choudhary PK, Goel A, Bhatia AK, Gururaj K, Gupta S, Singh SV. 2018. Immunomodulatory activity of Neolamarckia cadamba (Roxb.) Bosser with reference to IL-2 induction. Indian Journal of Traditional Knowledge, 17(3): 451-459.
  • 9 Audarya SD, Singh M, Stephen BJ, Sahzad, Chaubey KK, Gupta S, Pachoori A, Jayaraman S, Aseri GK, Sohal JS, Bhatia AK, Singh SV. 2018. Status of Johne's disease in buffalo population of Malwa region using goat based indigenous ELISA kit. Buffalo Bulletin. 37: 9-16.
  • 10 Chaubey KK, Singh SV, Bhatia AK, Gupta RD, Gupta S, Varshney A, Singh M, Singh MK, Hemati Z, Rathore A (2018). Detection Limits of Recombinant Secretary Proteins versus Semipurified Protoplasmic Antigens for the Diagnosis of Spontaneous Cases of Mycobacterium avium subspecies paratuberculosis Infection in Domestic Ruminants. J Vet Sci Technol 9: 552.
  • 11 Chaubey KK, Kumaresan G, Gupta RD, Singh SV, Bhatia AK, Rathore AS, Gupta S and Singh M (2018). Molecular Diversity and Homology in Six CFPs Genes in the Novel Bio-Type, 'Indian Bison Type' of Mycobacterium avium subspecies paratuberculosis of Goat Origin vs. other Biotypes. Mycobacterial Diseases. 8: 267. doi:10.4172/2161-1068.1000267.
  • 12 Matoli S , S.D. Audarya, D. Chhabra1, M. Singh, K. Chaubey, S. Gupta, R. Sikrodia, G.P. Jatav and S.V. Singh. 2018. Serological, Microscopical and Molecular Examinations of Cow Milk Samples for Diagnosis of Paratuberculosis at Mhow of Madhya Pradesh in India. International Journal of Current Microbiology and Applied Sciences, 7(11): 2153-2162.
  • 13 Singh SV, Gupta S, Chaubey KK, Bhushan S, Rawat KD, Kumar N, Tiwari HA, Chaturvedi V, Sohal JS, Dhama K and Hemati Z, 2017. 'Therapeutic Management' of Incurable Paratuberculosis Using 'Indigenous Vaccine' in Goatherds, Endemically Infected with Johne's Disease. International Journal of Pharmacology, 13: 145-155.
  • 14 Chaubey KK, Singh SV, Gupta S, Singh M, Sohal JS, Kumar N, Singh MK, Bhatia AK, Dhama K. (2017). Mycobacterium avium subspecies paratuberculosis – an important food borne pathogen of high public health significance with special reference to India: an update, Veterinary Quarterly, 37:1, 282-299.
  • 15 Biswal S, Pany SS, Singh SV, Sahoo N, Singh M, Gupta S, Chaubey KK. 2018. Bio-detection of Mycobacterium avium subspecies paratuberculosis in the Commercial Milk and Milk Products Sold in Bhubaneswar, Odisha, India. Int. J. Curr. Microbiol. App. Sci. 7(01): 753-761.
  • 16 Singh, M., Gupta, S., Singh, S.V., Chaubey, K.K., Sohal, J.S., Dhama, K. (2018) Bio-incidence of Mycobacterium avium subspecies paratuberculosis in the pasteurized liquid milk, flavoured milk and milk powder commercially sold by leading market brands in India. Journal of Experimental Biology and Agricultural Sciences (JEBAS) 6(1): 188-203.
  • 17 Mukartal SY, Rathannama D, Narayanswamy HD, Gupta S, Chaubey KK, Singh M, Hemati Z, Nishanth C, Pachoori A, Dhama K, Singh SV. 2017. Assessment of Ovine Johne's disease in the Mandya sheep breed in South India using multiple diagnostic tests and bio-typing of Mycobacterium avium subspecies paratuberculosis infection. Cogent Food & Agriculture (2017), 3: 1298391.
  • 18 Jayaraman S, Chaubey KK, Singh SV, Aseri GK, Mukta Jain, Gupta S, Singh M, Parul Yadav, Neeraj Khare, Deepansh Sharma, Sohal JS 2018. Pilot scale studies on the optimization of DIVA assay for the inactivated whole cell paratuberculosis vaccine. Int. J. Res. Pharm. Sci., 9(2), 554-565.
  • 19 Chaturvedi, S, Singh SV, Srivastava AK, Gangwar N, Kumar N, Rawat KD, Gupta S, Chaubey KK, Singh R, Raghavendra, Kuldeep D. (2017). Comparative evaluation of FAT, IS900 PCR and microscopy vis a vis histopathology for the detection of Mycobacterium avium subsp paratuberculosis infection in tissues of goats naturally died in herds endemic for Johne's disease. Indian Journal of Animal Sciences 87 (6): 18–00.
  • 20 Kuenstner JT, Naser S, Chamberlin W, Borody T, Graham DY, McNees A, Hermon-Taylor J, Hermon-Taylor A, Dow CT, Thayer W, Biesecker J, Collins MT, Sechi LA, Singh SV, Zhang P, Shafran I, Weg S, Telega G, Rothstein R, Oken H, Schimpff S, Bach H, Bull T, Grant I, Ellingson J, Dahmen H, Lipton J, Gupta S, et al., 2017. The Consensus from the Mycobacterium avium ssp. paratuberculosis (MAP) Conference 2017. Front. Public Health, 5:208.
  • 21 Mohan Kumar Chillahalli Mahalingaiah, Muralidhara Asoor, Ramesh Poojari Thimmaiah, Hogalagere Doddappaiah Narayanaswamy, Shivalingappa Yamanappa Mukartal, Anuradha Menon Elattuvalappil, Nishanth Chikkahonnaiah, Saurabh Gupta, Shoorvir Singh 2017.Prevalence of Canine Babesiosis in Different Breeds of Dogs in and Around Bengaluru. Advances in Animal and Veterinary Sciences 5(3): 140-144.
  • 22 Chaubey KK, Gupta RD, Gupta S, Singh SV, Bhatia AK, Jayaraman S, Kumar N, Goel A, Abhishek Singh Rathore AS, Sahzad, Sohal JS, BJ, Singh M, Goyal M, Dhama K & Derakhshandeh A 2016. Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock. Veterinary Quarterly, DOI: 10.1080/01652176.2016.1196508.
  • 23 Hasan K, Rathnamma D, Narayanaswamy HD, Malathi V, Tomar N, Gupta S, Singh SV. 2017. Current scenario and future perspectives of cpv-2 vaccines in india. Adv. Anim. Vet. Sci. 5(11): 446-448.
  • 24 Hasan K, Rathnamma D, Narayanaswamy HD, Malathi V, Gupta S, Singh SV (2017). Isolation of Bacterial Pathogens Associated with Broiler Mortality in Kolar. Adv. Anim. Vet. Sci. 5(7): 312-315.
  • 25 Shoor Vir Singh, Bjorn John Stephen, Manju Singh, Saurabh Gupta, Kundan Kumar Chaubey, Sahzad, Sujata Jayaraman, Gajendra Kumar Aseri, Jagdip Singh Sohal, Ashok Kumar Bhatia, Anjali Pachoori, Jitendra Chauhan and Kuldeep Dhama (2016). Evaluation of new 'indigenous milk dot-ELISA' as 'Field Test' vis-à-vis milk plate-ELISA for the detection of Mycobacterium avium sub species paratuberculosisinfection in lactating domestic livestock. Indian Journal of Biotechnology, 15(2): 166-171.
  • 26 Singh SV, Kuenstner JT, Davis WC, Agarwal P, Kumar N, Singh D, Gupta S, Chaubey KK, Tyagi AK, Misri J, Jayaraman S, Sohal JS and Dhama K (2016). Concurrent resolution of chronic diarrhea likely due to Crohn's disease and infection with Mycobacterium avium paratuberculosis. Front. Med. 3:49. http://dx.doi.org/10.3389/fmed.2016.00049.
  • 27 Garg R, Patil PK, Singh SV, Sharma S, Gandham RK, Singh AV, Filia G, Singh PK, Jayaraman S, Gupta S, Chaubey KK, Tiwari R, Saminathan M, Dhama K, Sohal JS. 2015. Comparative Evaluation of Different Test Combinations for Diagnosis of Mycobacterium avium subspecies paratuberculosis Infecting Dairy Herds in India. Biomed Research International, Article ID 983978, http://dx.doi.org/10.1155/2015/983978.
  • 28 Singh S.V., Singh P.K., Kumar N., Gupta S., Chaubey K.K., Singh B., Srivastav A., Yadav S. 2015. Evaluation of goat based 'Indigenous vaccine' against Bovine Johne's Disease in endemically infected native cattle herds. Indian Journal of Experimental Biology, Vol. 53, pp. 16-24.
  • 29 Sadana T., Singh R.V., Singh S.V., Saxena V.K., Sharma D., Singh P.K., Kumar N., Gupta S., Chaubey K.K., Jayaraman S., Tiwari R., Dhama K. and Sohal J.S. 2015. Single nucleotide polymorphisms of SLC11A1, CARD15, IFNG and TLR2 genes and their association with Mycobacterium avium subspecies paratuberculosis infection in native Indian cattle population. Indian Journal of Biotechnology, 14(4): 469-475.
  • 30 Singh S.V., Singh P.K., Singh A.V., Sohal J.S., Kumar N., Chaubey K.K., Gupta S., Kumar A., Bhatia A.K., Srivastav A.K., Dhama K. 2014. Bio-load and bio-type profiles of Mycobacterium avium subspecies paratuberculosis infection in the farm and farmer's herds / flocks of domestic livestock: A 28 years study (1985-2013). Transboundary and Emerging diseases, 61 (Suppl. 1): 43-55.
  • 31 Kumar N., Wadhwa A., Chaubey K.K., Singh S.V., Gupta S., Sharma S., Sharma D.K., Mishra A.K., Singh M.K. 2013. Isolation and phylogenetic analysis of an orf virus from sheep in Makhdoom, India. Virus Genes, DOI 10.1007/s11262-013-1025-9.
  • 32 Singh S.V., Kumar N., Singh S.N., Bhattacharya T., Sohal J.S., Singh P.K., Singh A.V., Singh B., Chaubey K.K., Gupta S., Sharma N., Kumar S., Raghava G.P.S. 2013. Genome Sequence of the “Indian Bison Type” Biotype of Mycobacterium avium subsp. paratuberculosis Strain S5. Genome Announcements (Microbiology Resource Announcements), 1(1), e00005-13.
  • 33 Singh S.V., Dhama, K., Chaubey, K.K., Kumar, N., Singh, P.K., Sohal, J.S., Gupta S., Singh, A.V., Verma, A.K., Tiwari, R., Mahima, S., Chakraborty and Deb, R. 2013. Impact of host genetics on susceptibility and resistance to Mycobacterium avium subspecies paratuberculosis infection in domestic ruminants. Pakistan Journal of Biological Sciences Vol. 16, Issue 6, pp. 251-266.
  • 34 Singh S.V., Singh A.V., Singh P.K., Gupta S., Singh H., Singh B., VinodhKumar OR, Rajendiran A.S., Swain N., Sohal J.S. 2013. Evaluation of 'Indigenous vaccine' developed using 'Indian Bison Type' genotype of Mycobacterium avium subspecies paratuberculosis strain 'S5' of goat origin in a sheep flock endemic for Johne's disease: A three years trial in India. World Journal of Vaccines, Vol. 3, Issue 2, pp. 52-59.
  • 35 Kumar A., Singh S.V., Srivastava A.K., Gangwar N.K., Singh P.K., Gupta S., Chaubey K.K. 2014. Comparative evaluation of 'indigenous' and commercial vaccines in double challenge model for the control of caprine paratuberculosis in India. Journal of Biological Sciences, Vol. 14, Issue 3, pp. 169-182.
  • 36 Singh S.V., Kumar N., Sohal J.S., Singh A.V., Singh P.K., Agrawal N.D., Gupta S., Chaubey K.K., Rawat K.D., Deb R., Dhama K. 2014. First mass screening of the human population to estimate the bio-load of Mycobacterium avium subspecies paratuberculosis in North India. Journal of Biological Sciences, Vol. 14, Issue 4, pp. 237-247.
  • 37 Pahangchopi D., Singh R.V., Singh S.V., Das P., Sharma D., Sardana T., Kumar N., Chaubey K.K., Gupta S. 2014. Evaluation of 'cattle' and 'Indian Bison' type antigens of Mycobacterium avium subspecies paratuberculosis for diagnosis of Bovine Johne's Disease using 'indigenous ELISA' and AGPT. Indian Journal of Experimental Biology, Vol. 52, pp. 1182-1185.
  • 38 Singh, S.V., Stephen, B.J., Singh, M., Gupta, S., Chaubey, K.K., Sahzad, Sachan T.K., Sohal, J.S., Dhama, K., Mukartal S.Y., Hemati Z., 2016. Comparison of newly standardized 'Latex milk agglutination test', with 'Indigenous milk ELISA' for 'on spot' screening of domestic livestock against Mycobacterium avium subsp. paratuberculosis infection. Indian J of Biotech. 15: 511-517.
  • 39 Singh, S.V., Stephen, B.J., Singh, M., Gupta, S., Chaubey, K.K., Sahzad, Jayaraman, S., Sachan, T.K., Aseri, G.K., Jain, M., Sohal, J.S., Dhama, K., 2016. Evaluation of Indirect Flurorescent Antibody Test (iFAT) as the 'mass screening test' for the diagnosis of Mycobacterium avium subspecies paratuberculosis infection in the milk samples of lactating domestic livestock. Journal of Experimental Biology & Agricultural Sciences. 4(5): 533-540.
  • 40 Singh S.V., Singh P.K., Gupta S., Chaubey K.K., Singh B., Kumar A., Singh A.V., Kumar N. 2014. Comparision of microscopy and blood-PCR for the diagnosis clinical of Johne's disease in domestic ruminant. Iranian Journal of Veterinary Research, 14, 345-349.
  • 41 Sohal JS, Singh SV, Singh B, Thakur S, Aseri GK, Jain N, Jayaraman S, Yadav P, Khare N, Gupta S, Chaubey KK, Dhama K (2015). Control of paratuberculosis: opinions and practices. Advances in Animal and Veterinary Sciences. Vol. 3, Issue 3, pp. 156-163.
  • 42 Singh S.V., Agarwal N.D., Kumar N., Gupta S., Chaubey K.K., Rawat K.D., Tiwari R., Sohal J.S., Sachan T.K., Sharma V., Dhama K., 2014. Screening of human population for Type 1 Diabetes and Mycobacterium avium subspecies paratuberculosis from Chattarpur district of Madhya Pradesh. Advances in Animal and Veterinary Sciences. Vol. 2, Issue 10.
  • 43 Barad D.B., Chandel B.S., Dadawala A.I., Chauhan H.C., Kher H.N., Shroff S., Bhagat A.G., Singh S.V., Singh P.K., Singh A.V., Sohal J.S., Gupta S., Chaubey K.K..2013. Comparative Potential of Traditional versus Modern Diagnostic Tests in Estimating Incidence of Caprine Johne's disease. Advances in Animal and Veterinary Sciences. Vol. 1, Issue 1, pp. 35-40.
  • 44 Shroff S., Chandel B.S., Dadawala A.I., Singh S.V., Bhagat A.G., Chauhan H.C., Gupta S. 2013. Evaluation of Indigenous vaccine in Patanwadi sheep naturally infected with clinical Johne's disease. Research Opinions in Animal & Veterinary Sciences, Vol. 3, Issue 9, pp. 322-329.
  • 45 Raghuvanshi T., Singh S.V., Sharma R.B., Gupta S., Chaubey K.K., Kumar N., Dhama K. 2013. A pilot study on the presence of Mycobacterium avium subspecies paratuberculosis in fresh cheese (paneer) prepared from goat milk of herds endemic for Johne's disease. Journal of Infection and Molecular Biology, Vol. 1, Issue 3, pp. 46-48.
  • 46 Singh S.V., Kumar N., Chaubey K.K., Gupta S., Rawat, K.D. 2013. Bio-presence of Mycobacterium avium subspecies paratuberculosis infection in domestic livestock farms. Research Opinions in Animal and Veterinary Sciences, Vol. 3, Issue. 11, pp. 401-406.
  • 47 Singh K., Chandel B.S., Dadawala A.I., Singh S.V., Chauhan H.C., Singh B., Agrawa N.D., Gupta S., Chaubey K.K. 2013. Incidence of Mycobacterium avium subspecies paratuberculosis in Mehsana Breed of Goats from North Gujarat using Multiple Tests. Advances in Animal and Veterinary Sciences. Vol. 1, Issue 1, pp. 28-31.
  • 48 Singh S.V., Singh P.K., Singh A.V., Gupta S., Chaubey K.K., Singh B., Kumar A., Srivastav A., Singh H., Sohal J.S. 2013. Bio-burden and bio-type profiles of Mycobacterium avium subspecies paratuberculosis infection in the domestic livestock population based on mass screenings using multiple tests. International Journal of Current Research, Vol. 5, Issue 7, pp. 1897-1901.
  • 49 Agrawal N.D., Agrawal K., Gupta S., Chaubey K.K., Shukla S., Mathur R. 2013. Therapeutic potential of Moringa oleifera root extract in attenuation of beryllium induced oxidative stress, biochemical alterations and histopathological lesions in rat. International Journal of Current Research, Vol. 5, Issue 7, pp. 1677-1683.
  • 50 Singh SV, Gupta S, Singh PK, Singh AV, Sohal JS, Kumar N, Kumar A, Chaubey KK and Singh B (2013). Therapeutic Management of Clinical Bovine Johne's disease Using Goat Based 'Indigenous Vaccine' in Native Hariana Cattle: Case Reports. Adv. Anim. Vet. Sci. 1 (1S):23 – 28.
  • 51 Singh S.V., Singh A. V., Gupta S., Rajindran A. S., Swain N., Singh P. K., Singh H., Sohal J. S., Kumar N. 2012. Interspecies Sharing of “Indian Bison Type,” A Novel predominant Genotype of Mycobacterium avium subspecies paratuberculosis between Naturally Infected and Endemic Flocks of Bharat Merino Sheep and a Colony of Rabbits (Oryctolagus Cuniculus) Raised on the Same Ecosystem in South India. Research and Reviews: A Journal of Life Sciences, Vol. 2, Issue 3, pp. 1-8.
  • 52 Audarya S.D., Chaubey K.K., Gupta S., Kumar N., Yadav D.S., Chhabra D., Sharda R., Garg U.K., Singh S.V. 2013. Investigation on Bovine Johne's Disease in an organized dairy farm of Mhow in Madhya Pradesh. International Journal of Current Research, Vol. 5, Issue, 10, pp. 2895-2897.
  • 53 Singh S.V., Tiwari A., Singh A.V., Singh P.K., Singh B., Kumar A., Gururaj K., Gupta S., Kumar N. 2012. Contamination of natural resources (soil and river water) with Mycobacterium avium subsp. paratuberculosis in three districts of Uttar Pradesh:a pilot study. Haryana Veterinarian, Vol.51, pp.1-5.
  • 54 Singh S.V., Chaubey K.K., Gupta S., Gupta V.K., Agarwal N.D., Kumar N. 2013. Co-infection of Mycobacterium avium subspecies paratuberculosis and Brucella melitensis in a Sirohi breed of goats in India. Advances in Animal and Veterinary Sciences, 1(6): 188-190.
  • 55 Singh S.V., Sohal J.S., Kumar N., Gupta S., Chaubey K.K., Rawat K.D., Chakraborty S., Tiwari R. and Dhama K. 2014. Recent approaches in diagnosis and control of mycobacterial infections with special reference to mycobacterium avium subspecies paratuberculosis. Advances in Animal and Veterinary Sciences, Vol. 2, Special Issue (IS): 1-11.
  • 56 Barad D.B., Chandel B.S., Dadawala A.I., Chauhan H.C., Kher H.N., Shroff S., Bhagat A.G., Singh S.V., Singh P.K., Singh A.V., Sohal J.S., Gupta S., Chaubey K.K. Chakraborty S., Tiwari R., Deb R., Dhama K.,.2014. Incidence of Mycobacterium avium subspecies paratuberculosis in Mehsani and Surti goats of Indian origin using multiple diagnostic tests. Journal of Biological Sciences, Vol. 14, Issue 2, pp. 124-133.
  • 57 Singh S.V., Gupta S., Chaubey K.K., Rawat K.D., Kumar N., Sohal J.S., Singh S., Tiwari R., Chakraborty S., Dhama K. 2014. Johne's disease (JD) in a high yielding Holstein Friesian cattle dairy farm in India. Journal of Biological Sciences, Vol. 14, Issue 3, pp. 195-203.
  • 58 Shroff S., Chandel B.S., Dadawala A.I., Gupta S., Chaubey K.K., Singh S. V. 2014. Incidence of Mycobacterium avium subspecies paratuberculosis in Patanwadi sheep. Indian Journal of Small Ruminants, Vol. 20, Issue 1, pp. 54-58.
  • 59 Singh S.V., Gupta S., Chaubey K.K., Kumar N., Rawat K.D., Tiwari R. and Dhama K. 2014. Dose Standardization Studies of 'Indigenous Vaccine' for the Control of Mycobacterium Avium Subspecies Paratuberculosis in Naturally Infected Goats. Advances in Animal and Veterinary Sciences, Vol. 2(IS), pp. 17-22.
  • 60 Singh S.V., Yadav R.K., Gupta V.K., Gupta S., Chaubey K.K., Agarwal N.D., Kumar N. 2014. Co-incidence of Bovine Johne's disease and Bovine Brucellosis in Young Bulls of Murrah Breed in their Native Tract (Rohtak, Haryana, India). Advances in Animal and Veterinary Sciences, Vol. 2(IS), pp. 23-25.
  • 61 Kumar T., Singh R.V., Sharma D., Gupta S., Chaubey K.K., Rawat K.D., Kumar N., Dhama K., Tiwari R., Singh S.V. 2014. Status of Mycobacterium avium subspecies paratuberculosis infection in an Indian goshala housing poorly or unproductive cows suffering with clinical Bovine Johne's disease. Advances in Animal and Veterinary Sciences, 2(5): 261-263.
  • 62 Singh S.V., Gupta V.K., Kumar A., Gupta S., Tiwari R. and Dhama K. (2014). Therapeutic Management of Bovine Brucellosis in Endemically Infected Dairy Cattle Herd of Native Sahiwal Breed. Advances in Animal and Veterinary Sciences, 2(1S): 32-36.
  • 63 Rawat KD, Chaudhary S, Kumar N, Gupta S, Chaubey KK, Singh SV, Dhama K, Verma AK, Deb R. (2014). Economic losses in a commercial dairy farm due to the out-break of Johne's disease in India. Research Journal for Veterinary Practitioners, 2 (5): 73-77.
  • 64 Rawat KD, Chaudhary S, Gupta S, Chaubey KK, Jayaraman S, Kumar N, Sohal JS, Sachan TK, Dhama K, Singh SV (2014). Potential of 'goat based vaccine' using 'India bison biotype' of Mycobacterium avium subspecies paratuberculosis in salvaging a dairy farm consisting of high yielding Holstein Fresian cows from devastation and closure due to outbreak of bovine Johne's disease in Northern India. Advances in Animal and Veterinary Sciences, 2(12): 638-646.
  • 65 B.J. Stephen, Mukta Jain, Kuldeep Dhama, S.V. Singh, Manali Datta, Neelam Jain, Sujata Jayaraman, Manju Singh, K.K. Chaubey, S. Gupta, G.K. Aseri, Neeraj Khare, Parul Yadav and J.S. Sohal (2015). Nanotechnology Based Therapeutics, Drug Delivery Mechanisms and Vaccination approaches for Countering Mycobacterium avium subspecies paratuberculosis (MAP) Associated Diseases. Asian Journal of Animal and Veterinary Advances. 10: 830-842.
  • 66 Singh B, Mukta Jain, Shoor Vir Singh, Kuldeep Dhama, G K Aseri, Neelam Jain, Manali Datta, Neeraj Khare, Parul Yadav, Sujata Jayaraman, Saurabh Gupta, Jagdip Singh Sohal*, Kundan kumar Chaubey (2015). Plants as Future Source of Anti-Mycobacterial Molecules and Armour for Fighting Drug Resistance. Asian Journal of Animal and Veterinary Advances. 10: 443-460.
  • 67 Shoor Vir Singh, Sachin Digambar Audarya, Manju Singh, Bjorn John Stephen, Daljeet Chhabra, Kundan Kumar Chaubey, Saurabh Gupta, Sahzad, Anjali Pachoori, Sujata Jayaraman, Gajendra Kumar Aseri, Jagdip Singh Sohal, Ashok Kumar Bhatia and Kuldeep Dhama (2015). Development of New 'Indigenous Dot-ELISA Kit' as Sensitive Field Based Herd Screening Test for the Diagnosis of Johne's Disease in the Domestic Buffalo Population. Asian Journal of Animal and Veterinary Advances. DOI: 10.3923/ajava.2016.
  • 68 Bjorn J Stephen, Manju Singh, Saurabh Gupta, Kundan K Chaubey, Sahzad, Sujata Jayaraman,Mukta Jain, Jagdip S Sohal, Kuldeep Dhama (2015). High 'Bio-contamination' of milk products (Fresh cheese or Paneer) with Mycobacterium avium subspecies paratuberculosis sold by local dairies in rural and urban area of South Uttar Pradesh (North India) using six diagnostic tests. Advances in Animal and Veterinary Sciences.
  • 69 Sachan T.K., Singh S.V., Virendra Kumar, Gupta S., Chaubey K.K., Sujata Jayaraman, Mukesh Sikarwar, Sunil Dixit, Dhama K. 2015. Chemical and Ultrastructural characteristics of mycobacterial biofilms. Asian Journal of Animal and Veterinary advances Volume 10, Number 10, 592-622, 2015.
  • 70 Hasan K, Rathnamma D, Narayanaswamy HD, Isloor S, Chandranaik BM, Kshama MA, Elattuvalappil AM, Mukartal SY, Singh SV, Gupta S and Krishnappa S. 2016. Comparative evaluation of lateral flow assay and PCR for detection of canine parvovirus. Advances in Animal and Veterinary Sciences 4(11): 580-583.
  • 71 Sahzad, Singh SV, Gupta S, Chaubey KK, Singh M, Dayal, Deen, Dhama K, Hemati, Z, Srivastava M, Mansi. 2017. Comparison of Antibody Response and Direct Microscopy between Male and Female Goats in Response to Infection of Mycobacterium avium subspecies paratuberculosis in Goatherds Endemic for Johne's Disease. JSM microbiology. 5. 1034.
  • 72 Sahzad, Singh SV, Chaubey KK, Gupta S, Kumar, P, Kumar R, Pratap Singh, Akhand. 2017. A New Rapid and Cost Effective Genomic DNA Extraction Method for Molecular Diagnosis of Mycobacterium avium subspecies paratuberculosis from Various Sources. JSM Microbiology. 5. 1036.
  • 73 Krishnappa S, Satyanarayana ML, Rao S, Yathiraj S, Shridhar NB, Mukartal SY, Gupta S, Singh S, Dhama K, Elattuvalappil AM (2016). Pathomorphological studies on the effect of aged garlic extract in aflatoxin B1 induced liver damage in wistar albino rats. Adv. Anim. Vet. Sci. 4(12): 648-654.
  • 74 Audarya, S.D., Chhabra D, Sahzad, Singh M, Stephan BJ, Chaubey KK, Gupta S, Sohal JS, Dhama K, Mukartal SY, Rathnamma D, Singh SV, 2016. Bio-incidence of bovine Johne's disease in dairy buffaloes in Central and North India using sensitive goat based 'indigenous ELISA kit' and traditional tests. J. Exp. Biol. Agric. Sci., 4: 525-532.
  • 75 Krishnappa S, Satyanarayana ML, Narayanaswamy HD, Rao S, Yathiraj S, Isloor S, Mukartal SY, Gupta S, Singh SV, Elattuvalappil AM, Dhama K, Mallaiah S (2016). Haemato-biochemical profile and milk leukocyte count in subclinical and clinical mastitis affected buffaloes. Adv. Anim. Vet. Sci. 4(12): 642-647.

Genome and Nucleotide Sequences Published in NCBI: 09

  • Genome Sequence of the 'Indian Bison Type' Biotype of Mycobacterium avium subspecies paratuberculosis Strain 'S5' under genomeA00005-13.
  • Kumar, N., Wadhwa, A., Chaubey, K.K., Singh, S.V., Gupta, S., Sharma, S., Sharma, D.K., Singh, M.K. and Mishra, A.K. Orf virus isolate CIRG major envelope protein gene. Accession No. KC992325.
  • Kumar N., Kachhawa S., Kashyap S.K., Maherchandani S., Singh S.V., Chaubey K.K., Gupta, S. and Rawat K.D. Peste-des-petits-ruminants virus strain Nagaur1 fusion protein mRNA, complete cds isolated from outbreak in Nagaur, Rajasthan. Accession No. KJ081283.
  • Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Kumar,N., Gupta,S., Singh,M. and Bhatia,A.K. Mycobacterium avium subsp. paratuberculosis strain S5 peptidyl-prolyl cis-trans isomerase gene, partial cds. GenBank: MG753462.1.
  • Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Kumar,N., Gupta,S., Singh,M. and Bhatia,A.K. Mycobacterium avium subsp. paratuberculosis strain S5 putative low molecular weight antigen gene, partial cds. GenBank: MG753463.1
  • Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Bhatia,A.K., Kumar,N., Gupta,S. and Singh,M. Mycobacterium avium subsp. paratuberculosis strain S5 ModD (modD) gene, partial cds. GenBank: MG753464.1
  • Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Bhatia,A.K., Kumar,N., Gupta,S. and Singh,M. Mycobacterium avium subsp. paratuberculosis strain S5 secreted fibronectin-binding protein C antigen 85-C (fbpC) gene, partial cds. GenBank: MG753465.1
  • Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Bhatia,A.K., Kumar,N., Gupta,S. and Singh,M. Mycobacterium avium subsp. paratuberculosis strain S5 serine protease PepAN (pepAN) gene, partial cds. GenBank: MG753466.1
  • Chaubey,K.K., Singh,S.V., Gururaj,K., Gupta,R.D., Rathore,A.S., Bhatia,A.K., Kumar,N., Gupta,S. and Singh,M. Mycobacterium avium subsp. paratuberculosis strain S5 serine protease PepAC (pepAC) gene, partial cds. GenBank: MG753467.1

Contributory Lead Paper / Abstracts at International Conference: 28

  • In the 14th International Colloquium on Paratuberculosis (ICP), Riviera Maya, Mexico (4-8,June,2018)-2
  • Lead Paper in 1st International Satellite Symposium (ISS)-2016 on "Advances in Animal Sciences & Biomedicine in 21st Century", 12th Oct. 2016 in UK -01
  • Abstracts in 1st International Satellite Symposium (ISS)-2016 on "Advances in Animal Sciences & Biomedicine in 21st Century", 12th Oct. 2016 in UK -03
  • In the 12th International Conference on Goats (IGA), Antalya, Turkey (25-30, Sep., 2016) -03
  • In the 13th International Colloquium on Paratuberculosis (ICP), Nantes, France (20-24, June, 2016) - 05
  • In the 12th International Colloquium on Paratuberculosis (ICP), Parma, Italy (22-26, June, 2014) - 07
  • In the 11th International Colloquium on Paratuberculosis (ICP), Sydney, Australia (5-10, Feb, 2012) - 06
  • In the JDIP 7th Annual Conference, New Orleans, Louisiana, USA (10-14 July, 2011) - 02

Contributory Abstracts at National Conference: 18

  • In workshop on 'Commercial and Backyard Goat Farming with Scientific Approach' during the 5th Rajasthan Science Congress held at the Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur,13-14, Oct., 2017 - 01
  • In International conference on Nanotechnology in Energy, Nano-bio interface & Sustainable Environment (INTENSE-2017) held at the Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur on 19-21 August, 2017 - 01
  • In: Compendium of XXV National Conference of Veterinary Parasitology and National Symposium on “One Health Approach-Plausible solution for sustainable parasite control” held at MVC, TANUVAS, Chennai on 16-19th feb, 2016 - 01
  • In 16th Indian Veterinary Congress and XXIII Annual Conference of IAAVR and National Symposium on Strengthening of governance in animal health and production activities for benefit of farmers and livestock owners organized by Department of Veterinary Clinical Medicine, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and technology, Bhubaneswar from 27th to 28th February, 2016- 01
  • In World Veterinary Day-Shalihotra Jayanti and National Symposium on One Health Initiative to Foster Intersectoral Collaboration for Human and Animal Health organized by Department of Veterinary Public Health and Epidemiology, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar from 26th to 28th April, 2016- 02
  • XXVIII Annual Convention of Indian Association of Veterinary Microbiologists, Immunologists and specialists in Infectious Diseases (IAVMI) and International conference on "Challenges and opportunities in Animal Health at the face of globalization and climate change" held at DUVASU, Mathura during Oct. 30-Nov.1, 2014 - 04
  • In National conference on Zoonotic mycobacterial infection and their impact on public health, organized by Dept. of Biotechnology, held at All India Institute of Medical Sciences, New delhi, during Feb. 25-27, 2013 - 01
  • In National Conference on "Phytomedicine-An Alternate to Modern Medicine and Their Molecular Expression" organized by Dept. of Biotechnology and Pharmacy, GLA University, Mathura during, Feb. 16-17, 2013 - 02
  • In the compendium of National Seminar on "Future challenges and opportunities to improve health and production of small ruminants" and Annual Conference of Indian Society for Sheep and Goat Production and Utilization (ISSGPU) held at Central Institute for Research on Goats, Makhdoom, Mathura, U.P., during Dec. 22-23, 2012 - 05

Report/ Books/ Chapters: 05

S.No. Title Author's Name Publisher Year of Publication
1. Laboratory Manual (Pages 280) on isolation, identification and characterization of Mycobacterium avium subspecies paratuberculosis in domestic livestock, wild animals, milk and milk products, environment samples and human beings. Developed under the project- 'Development of nano-immuno rapid test to detect Mycobacterium avium subspecies paratuberculosis in milk samples'. Singh S.V., Gururaj K., Singh M., Gupta S., Chaubey K.K., Sohal J.S., Hemati Z., Funded by Ministry of Food Processing Industries, Govt. of India, New Delhi (SERB-MOFPI) 2017
2. Project Report of ICAR funded project entitled "Outreach Programme on Zoonotic Diseases" upto 31st March 2017 Singh S.V. & Gupta S. Funded by ICAR, New Delhi 2017
3. 'Bio-load' and bio-type profiles of Mycobacterium avium subspecies paratuberculosis in the biotic and abiotic environment of the country: A 28 years study (1985-2013) S.V. Singh, P.K. Singh, A.V. Singh, J.S. Sohal, N. Singh, N. Kumar, H.A. Tiwari, K.K. Chaubey, S. Gupta & A. Kumar. Funded by CSIR-NMITLI 2015
4. Project Report of NMITLI project on "Development and characterization of Indigenous Vaccine and diagnostics for Johne's Disease" completed on 31st Dec., 2014 S.V. Singh, S. Gupta & K.K. Chaubey Funded by CSIR-NMITLI 2014
5. Impact of host genetics on the control of Mycobacterium avium subspecies paratuberculosis infection in domestic livestock. In National seminar and Annual conference on Future challenges and Opportunities to Improve Health and Production of Small Ruminants (ISSGPU), Chapter 15, page: 255-280. S.V. Singh, K.K. Chaubey, N. Kumar, P.K. Singh, J.S. Sohal & S. Gupta Satish Serial Publishing House, Azadpur Delhi. 2012

Work Experience: 9.5 Years

In GLA University, Mathura:

  • Senior Research Officer at Department of Biotechnology (Since 8th Sept., 2018 - cont.)

In Central Institute for Research on Goats, Makhdoom:

  • Senior Research Fellow in ICAR_NASF funded project entitled "Identification of Biomarkers for Early Diagnosis of MAP Infection & Development of DIVA test"(11 months 20 days).
  • Research Associate in ICAR funded project entitled "Outreach Programme on Zoonotic Diseases" (2 years 1 month).
  • Research Associate in CSIR-NMITLI project entitled "Development and characterization of Indigenous Vaccine and diagnostics for Johne's Disease" (4 years 2 months).
  • Teaching to trainees (UG & PG students) coming for training in Health and diseases.

In National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra:

  • Project Assistant in National JALMA Institute for Leprosy and Other Mycobacterial Diseases, Agra, India in DBT project entitled "Drug Resistance Surveillance of Anti-tuberculosis Drugs in 35 district of Western UP and Bundelkhand region" (1 year 8 months).

Awards: 04

2016: ‘NRDC Societal Innovation award, 2014’ for innovation entitled ‘Indigenous vaccine against Johne’s Disease in domestic livestock’.
2014: International Association for Paratuberculosis (IAP) ‘Helping Hand Award’ to attend and present the work at 12th International Colloquium on Paratuberculosis (ICP), Parma, Italy.
2014: ‘Certificate of Honour’ for the Best Research article published in Intas Polivet,15(1), 2014. 2017: Best Poster Presentation Award at ISSGPU 2017 at ICAR-CIRG, Makhdoom, Mathura
2012: Qualified UPCET- 2012

Research Interest:

Major Areas-(Microbiology and Molecular Epidemiology)

  • Microbiology and Immunology (Study microbiome, transcriptome and immune interactions in Johne’s disease and Crohn’s disease with reference to M. paratuberculosis)
  • Molecular Epidemiology (Isolation, characterization and genotyping)
  • Proteomics (Immunogenicity of secretory proteins)

Technical Skills:

  • Microbiological Techniques
  • Immunological techniques:ELISA variants (Direct & Indirect)
  • Molecular Techniques: Microbial DNA & RNA isolation, RFLP, RAPD, Spoligotyping, MIRU-VNTR Real Time PCR, Electrophoresis and Blotting Techniques
  • Experience of working in BSL II and BSL III  GLP & ISO certified laboratories

Project Training:

Dissertation on “Molecular Characterization of Mycobacterium tuberculosis isolates by RAPD Analysis from Lohamandi Slum Area of Agra” under the supervision of Dr. V.D. Sharma, Deputy Director and Dr. V.M. Katoch, the then Director of National JALMA Institute of Leprosy & Other Mycobacterial Diseases, Agra as Co-Supervisor.

Scientific Affiliations:

  • Founder Member, International Academy of Biosciences (IAB), UK (2016-onward)
  • Member, International Association for Paratuberculosis, USA (2014-onward).

Other Activities and Achievements:

  • Attended Workshop on "Intellectual Property Rights: Patents and Copyrights" on Sept. 9-10, 2017 at GLA University, Mathura.
  • Best Poster Presentation Award at ISSGPU, 2017 at ICAR-CIRG, Makhdoom, Farah, Mathura
  • Qualified UPCET- 2012
  • Attended the CPCSEA Workshop on Animal Ethics Issues organized by Animal Experimentation Facility, National JALMA Institute for Leprosy & Other Mycobacterial Diseases, Agra.
  • Presented Poster at 12th International colloquium on Paratuberculosis, at Parma, Italy on 22-26th June, 2014.
  • Presented Poster at XXVIII Annual Convention of Indian Association of Veterinary Microbiologists, Immunologists and specialists in Infectious Diseases (IAVMI) at DUVASU, Mathura on Oct. 30- Nov.1, 2014.
  • Presented Poster at National conference on Phytomedicines, at GLA, University, Mathura.
  • Presented Poster on World TB Day in JALMA Institute of Leprosy & OMD, Tajganj, Agra.

References:

1. Dr. S.V. Singh
Prof. & Head
Dept. of Biotechnology, GLAUniversity,
Chaumuhan, Mathura- 281406
email: shoorvir_singh@rediffmail.com

2. Dr. A.K. Bhatia
ProfessorEmeritus,
Dept. of Biotechnology, GLA University
Mathura- 281406, UttarPradesh
email: akbhatia33@yahoo.com

3. Dr. Naveen Kumar
Principal Scientist,
VTCC, ICAR-NRC on Equines, Hisar, Haryana
email: naveenkumar.icar@gmail.com