Production and validation of Indigenous Johnin from native strain Indian Bison
Project Name Production and validation of Indigenous Johnin from native strain Indian Bison Type of Mycobacterium avium subsp. paratubarculosis for the diagnosis of Johne's disease in domestic livestock as the field test.
Funding Agency NRDC, New Delhi
Amount 2.0 lakhs
Cordinator & PI Dr. Shoor Vir Singh, Department of Biotechnology, GLA University, Mathura
Co-PI Dr. Saurabh Gupta & Dr. Kundan K. Chaubey, Department of Biotechnology, GLA University, Mathura
Duration 1 Year (2019-2020) Ongoing
Summary
Paratuberculosis or Johne's disease is a chronic debilitating infectious disease of domestic livestock leading to major production losses caused by Mycobacterium avium subspecies paratuberculosis (MAP). Estimated >70.0% of US dairy cattle herds are infected with JD, costing between $200 million and $1.5 billion per year to US dairy industry. In India, studies on losses incurred due to JD by way of increased mortality and morbidity and reduced productivity are non-existent. Severity of the disease was compounded by persistent chronic asymptomatic infectious nature of MAP leading to silent (latent) or sub-clinical stage (Stage 1). Intra-dermal 'Johnin test' has been the only 'field test' available for now more than half century for the detection of animals infected with MAP using Purified Protein Derivatives (PPDs). Johnin produced using imported strain resulted in lot of controversies with respect to specificity and sensitivity. Therefore, in the absence of efficient field test there was huge setback to the National Johne's disease control programs. Therefore, present study proposes to produce and validate Johnin or PPD from our native and fully characterized strain (S 5) of MAP biotype 'Indian Bison Type' the major bio-type prevalent and reported from wide variety of agro-climatic regions of the country.